22-Nucleotide RNAs trigger secondary siRNA biogenesis in plants.
Identifieur interne : 000620 ( Main/Exploration ); précédent : 000619; suivant : 00062122-Nucleotide RNAs trigger secondary siRNA biogenesis in plants.
Auteurs : Ho-Ming Chen [Taïwan] ; Li-Teh Chen ; Kanu Patel ; Yi-Hang Li ; David C. Baulcombe ; Shu-Hsing WuSource :
- Proceedings of the National Academy of Sciences of the United States of America [ 1091-6490 ] ; 2010.
Descripteurs français
- KwdFr :
- ARN des plantes (biosynthèse), ARN des plantes (génétique), Arabidopsis (génétique), Arabidopsis (métabolisme), Extinction de l'expression des gènes (MeSH), Petit ARN interférent (biosynthèse), Petit ARN interférent (génétique), Plantes (génétique), Plantes (métabolisme), Rhizobium (génétique), Similitude de séquences d'acides nucléiques (MeSH), Séquence nucléotidique (MeSH), Tabac (génétique), Tabac (métabolisme), Végétaux génétiquement modifiés (MeSH), microARN (biosynthèse), microARN (génétique).
- MESH :
- biosynthèse : ARN des plantes, Petit ARN interférent, microARN.
- génétique : ARN des plantes, Arabidopsis, Petit ARN interférent, Plantes, Rhizobium, Tabac, microARN.
- métabolisme : Arabidopsis, Plantes, Tabac.
- Extinction de l'expression des gènes, Similitude de séquences d'acides nucléiques, Séquence nucléotidique, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- Arabidopsis (genetics), Arabidopsis (metabolism), Base Sequence (MeSH), Gene Silencing (MeSH), MicroRNAs (biosynthesis), MicroRNAs (genetics), Plants (genetics), Plants (metabolism), Plants, Genetically Modified (MeSH), RNA, Plant (biosynthesis), RNA, Plant (genetics), RNA, Small Interfering (biosynthesis), RNA, Small Interfering (genetics), Rhizobium (genetics), Sequence Homology, Nucleic Acid (MeSH), Tobacco (genetics), Tobacco (metabolism).
- MESH :
- chemical , biosynthesis : MicroRNAs, RNA, Plant, RNA, Small Interfering.
- genetics : Arabidopsis, MicroRNAs, Plants, RNA, Plant, RNA, Small Interfering, Rhizobium, Tobacco.
- metabolism : Arabidopsis, Plants, Tobacco.
- Base Sequence, Gene Silencing, Plants, Genetically Modified, Sequence Homology, Nucleic Acid.
Abstract
The effect of RNA silencing in plants can be amplified if the production of secondary small interfering RNAs (siRNAs) is triggered by the interaction of microRNAs (miRNAs) or siRNAs with a long target RNA. miRNA and siRNA interactions are not all equivalent, however; most of them do not trigger secondary siRNA production. Here we use bioinformatics to show that the secondary siRNA triggers are miRNAs and transacting siRNAs of 22 nt, rather than the more typical 21-nt length. Agrobacterium-mediated transient expression in Nicotiana benthamiana confirms that the siRNA-initiating miRNAs, miR173 and miR828, are effective as triggers only if expressed in a 22-nt form and, conversely, that increasing the length of miR319 from 21 to 22 nt converts it to an siRNA trigger. We also predicted and validated that the 22-nt miR771 is a secondary siRNA trigger. Our data demonstrate that the function of small RNAs is influenced by size, and that a length of 22 nt facilitates the triggering of secondary siRNA production.
DOI: 10.1073/pnas.1001738107
PubMed: 20643946
PubMed Central: PMC2930544
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Baulcombe</name></author><author><name sortKey="Wu, Shu Hsing" sort="Wu, Shu Hsing" uniqKey="Wu S" first="Shu-Hsing" last="Wu">Shu-Hsing Wu</name></author></analytic><series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title><idno type="eISSN">1091-6490</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Arabidopsis (genetics)</term><term>Arabidopsis (metabolism)</term><term>Base Sequence (MeSH)</term><term>Gene Silencing (MeSH)</term><term>MicroRNAs (biosynthesis)</term><term>MicroRNAs (genetics)</term><term>Plants (genetics)</term><term>Plants (metabolism)</term><term>Plants, Genetically Modified (MeSH)</term><term>RNA, Plant (biosynthesis)</term><term>RNA, Plant (genetics)</term><term>RNA, Small Interfering (biosynthesis)</term><term>RNA, Small Interfering (genetics)</term><term>Rhizobium (genetics)</term><term>Sequence Homology, Nucleic Acid (MeSH)</term><term>Tobacco (genetics)</term><term>Tobacco (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN des plantes (biosynthèse)</term><term>ARN des plantes (génétique)</term><term>Arabidopsis (génétique)</term><term>Arabidopsis (métabolisme)</term><term>Extinction de l'expression des gènes (MeSH)</term><term>Petit ARN interférent (biosynthèse)</term><term>Petit ARN interférent (génétique)</term><term>Plantes (génétique)</term><term>Plantes (métabolisme)</term><term>Rhizobium (génétique)</term><term>Similitude de séquences d'acides nucléiques (MeSH)</term><term>Séquence nucléotidique (MeSH)</term><term>Tabac (génétique)</term><term>Tabac (métabolisme)</term><term>Végétaux génétiquement modifiés (MeSH)</term><term>microARN (biosynthèse)</term><term>microARN (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>MicroRNAs</term><term>RNA, Plant</term><term>RNA, Small Interfering</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>ARN des plantes</term><term>Petit ARN interférent</term><term>microARN</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Arabidopsis</term><term>MicroRNAs</term><term>Plants</term><term>RNA, Plant</term><term>RNA, Small Interfering</term><term>Rhizobium</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN des plantes</term><term>Arabidopsis</term><term>Petit ARN interférent</term><term>Plantes</term><term>Rhizobium</term><term>Tabac</term><term>microARN</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Arabidopsis</term><term>Plants</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Arabidopsis</term><term>Plantes</term><term>Tabac</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Gene Silencing</term><term>Plants, Genetically Modified</term><term>Sequence Homology, Nucleic Acid</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Extinction de l'expression des gènes</term><term>Similitude de séquences d'acides nucléiques</term><term>Séquence nucléotidique</term><term>Végétaux génétiquement modifiés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The effect of RNA silencing in plants can be amplified if the production of secondary small interfering RNAs (siRNAs) is triggered by the interaction of microRNAs (miRNAs) or siRNAs with a long target RNA. miRNA and siRNA interactions are not all equivalent, however; most of them do not trigger secondary siRNA production. Here we use bioinformatics to show that the secondary siRNA triggers are miRNAs and transacting siRNAs of 22 nt, rather than the more typical 21-nt length. Agrobacterium-mediated transient expression in Nicotiana benthamiana confirms that the siRNA-initiating miRNAs, miR173 and miR828, are effective as triggers only if expressed in a 22-nt form and, conversely, that increasing the length of miR319 from 21 to 22 nt converts it to an siRNA trigger. We also predicted and validated that the 22-nt miR771 is a secondary siRNA trigger. Our data demonstrate that the function of small RNAs is influenced by size, and that a length of 22 nt facilitates the triggering of secondary siRNA production.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20643946</PMID><DateCompleted><Year>2010</Year><Month>09</Month><Day>23</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>11</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1091-6490</ISSN><JournalIssue CitedMedium="Internet"><Volume>107</Volume><Issue>34</Issue><PubDate><Year>2010</Year><Month>Aug</Month><Day>24</Day></PubDate></JournalIssue><Title>Proceedings of the National Academy of Sciences of the United States of America</Title><ISOAbbreviation>Proc Natl Acad Sci U S A</ISOAbbreviation></Journal><ArticleTitle>22-Nucleotide RNAs trigger secondary siRNA biogenesis in plants.</ArticleTitle><Pagination><MedlinePgn>15269-74</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1073/pnas.1001738107</ELocationID><Abstract><AbstractText>The effect of RNA silencing in plants can be amplified if the production of secondary small interfering RNAs (siRNAs) is triggered by the interaction of microRNAs (miRNAs) or siRNAs with a long target RNA. miRNA and siRNA interactions are not all equivalent, however; most of them do not trigger secondary siRNA production. Here we use bioinformatics to show that the secondary siRNA triggers are miRNAs and transacting siRNAs of 22 nt, rather than the more typical 21-nt length. Agrobacterium-mediated transient expression in Nicotiana benthamiana confirms that the siRNA-initiating miRNAs, miR173 and miR828, are effective as triggers only if expressed in a 22-nt form and, conversely, that increasing the length of miR319 from 21 to 22 nt converts it to an siRNA trigger. We also predicted and validated that the 22-nt miR771 is a secondary siRNA trigger. Our data demonstrate that the function of small RNAs is influenced by size, and that a length of 22 nt facilitates the triggering of secondary siRNA production.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Ho-Ming</ForeName><Initials>HM</Initials><AffiliationInfo><Affiliation>Institute of Plant and Microbial Biology, Academia Sinica, Taipei 11529, Taiwan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Li-Teh</ForeName><Initials>LT</Initials></Author><Author ValidYN="Y"><LastName>Patel</LastName><ForeName>Kanu</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Yi-Hang</ForeName><Initials>YH</Initials></Author><Author ValidYN="Y"><LastName>Baulcombe</LastName><ForeName>David C</ForeName><Initials>DC</Initials></Author><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Shu-Hsing</ForeName><Initials>SH</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>233325</GrantID><Agency>European Research Council</Agency><Country>International</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2010</Year><Month>07</Month><Day>19</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Proc Natl Acad Sci U S A</MedlineTA><NlmUniqueID>7505876</NlmUniqueID><ISSNLinking>0027-8424</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C534587">MIRN173 microRNA, Arabidopsis</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance 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IdType="pubmed">17400893</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Taïwan</li></country></list><tree><noCountry><name sortKey="Baulcombe, David C" sort="Baulcombe, David C" uniqKey="Baulcombe D" first="David C" last="Baulcombe">David C. Baulcombe</name><name sortKey="Chen, Li Teh" sort="Chen, Li Teh" uniqKey="Chen L" first="Li-Teh" last="Chen">Li-Teh Chen</name><name sortKey="Li, Yi Hang" sort="Li, Yi Hang" uniqKey="Li Y" first="Yi-Hang" last="Li">Yi-Hang Li</name><name sortKey="Patel, Kanu" sort="Patel, Kanu" uniqKey="Patel K" first="Kanu" last="Patel">Kanu Patel</name><name sortKey="Wu, Shu Hsing" sort="Wu, Shu Hsing" uniqKey="Wu S" first="Shu-Hsing" last="Wu">Shu-Hsing Wu</name></noCountry><country name="Taïwan"><noRegion><name sortKey="Chen, Ho Ming" sort="Chen, Ho Ming" uniqKey="Chen H" first="Ho-Ming" last="Chen">Ho-Ming Chen</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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